Csf1 Rat Monoclonal Antibody [Clone ID: ER-MP58]

CAT#: BM4089

Csf1 rat monoclonal antibody, clone ER-MP58, Partially Purified

Product Images

BM4089 ER-MP58 antibody staining of Mouse Spleen Frozen Section.

Specifications

Product Data

• Clone Name: ER-MP58
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1/20-1/50.
  Immunohistochemistry on Paraffin Sections: 1/20 (Protein Kinase pretreatment for antigen retrieval is recommended).
  Has been described to work in FACS.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Mouse macrophage cell lines.
  Antigen/Epitope: Nature and function of the surface localized antigen are unknown. The antigen failed to immunoprecipitate.
• Specificity: This Monoclonal Antibody (Clone ER-MP58) detects all M-CSF responsive cells in the bone marrow and the majority of other myeloid blood cell precursors. The antigen is detected on a broad development range of macrophage precursor cells up to the monocytic level. The antibody is also very suitable for the identification of myeloid haematopoietic islands in various organs, and for embryonic studies.
  Antigen Distribution
  Isolated cells: The antigen is present on bone marrow myeloid cells (over 60% of freshly isolated bone marrow cells) including all M-CSF responsive cells. On myeloid cell lines the antigen is highly expressed in cells showing characteristics of cell precursors. Expression of the antigen decreases with maturation of macrophages.
  Tissue Sections: The antigen can be detected in all tissues containing myelopoietic islands.
• Formulation: PBS, pH 7.2
  State: Partially Purified
  State: Liquid partially purified Cell Culture Supernatant
  Preservative: 0.05% Sodium Azide
• Storage: Store undiluted at 2-8°C for one month or (in alqiuots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 12977 Mouse
• Synonyms: CSF1, MCSF, Macrophage colony-stimulating factor 1, Macrophage Colony Stimulating Factor