Csf1 Rat Monoclonal Antibody [Clone ID: ER-MP58]
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Product Data | |
Clone Name | ER-MP58 |
Applications | FC, IHC |
Recommended Dilution | Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50. Flow cytometry: The typical starting working dilution is 1:50. |
Reactivities | Mouse |
Host | Rat |
Isotype | IgG2a |
Clonality | Monoclonal |
Specificity | The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells ut to the monocytic level, but also on grnaulocytes. ERxpression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commenly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hemopoietic islands in various organs, and for embryonal studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 and ER-MP-20. |
Formulation | PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide |
Concentration | 0.1 mg/ml |
Purification | Protein G |
Database Link | |
Synonyms | CSF1, MCSF, Macrophage colony-stimulating factor 1, Macrophage Colony Stimulating Factor |
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