Gbgt1 (Forssman antigen) Rat Monoclonal Antibody [Clone ID: FOM-1]

CAT#: BM4091

Gbgt1 (Forssman antigen) rat monoclonal antibody, clone FOM-1, Purified

Specifications

Product Data

• Clone Name: FOM-1
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/1000).
  Immunohistochemistry on Paraffin Sections: 5 μg/ml (1/100). Pretreatment not necessary.
  Suggested Positive Control: Mouse spleen.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgM
• Clonality: Monoclonal
• Specificity: The Monoclonal antibody FOM-1 detects the Forssman antigen.
  The FOM-1 antigen is a glycosphingolipid (FO GSL) differentiation antigen.
  Antigen Distribution:
  In tissue sections the antigen is found on macrophages in the red pulp of the spleen and the medulla of inguinal and axial lymph nodes. It's negative on monocytes and granulocytes. Reticular cells in T-cell areas of spleen and lymph nodes also stain positively. In response to inflammatory stimuli, a subpopulation of positive macrophages appear in the peritoneal cavity. Other macrophages in lymphoid and non lymphoid organs (e.g. alveolar macrophages and Kupffer cells) are negative.
  These results indicate that infiltrating macrophages carry the antigen.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: > 0.5 mg/ml
• Purification: Partially purified by Dialysis and Gel Filtration
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 227671 Mouse
• Background: The Forssman antigen was named after the Swedish pathologist John F. Forssman and later identified as the GalNAcα(1-3)GalNAcβ(1-R) disaccharide group. Forssman specificity was described in many animal species, plants and bacteria. In the mouse, Forssman antigen is a developmental and differentiation antigen. Expression of Forssman antigen in macrophages can be modulated by cytokines.
• Synonyms: A3GALNT; FS; MGC44848; UDP-GalNAc; UNQ2513