MHC Class I H-2 Dd, H-2(k,q,s) Rat Monoclonal Antibody [Clone ID: ER-MP42]

CAT#: BM4092

MHC Class I H-2 Dd, H-2(k,q,s) rat monoclonal antibody, clone ER-MP42, Purified

Product Images

Antigen distribution: The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.

Specifications

Product Data

• Clone Name: ER-MP42
• Applications: Assay, FC, IHC
• Recommended Dilution: Immunohistology on frozen sections (1:200; Acetone, Formaldehyde, Glutaraldehyde are suitable fixatives).
  Does not react on routinely processed paraffin sections.
  FACS (1:50 - 1:100).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse macrophage precursor cells
• Specificity: Monoclonal antibody ER-MP42 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd, H-2k,q,s. A weaker reactivity is found in mouse strains with the following haplotypes: H-2p,r,w7,w22.
  MHC class I molecules of other haplotypes are not recognized by ER-MP42.
• Formulation: PBS, pH 7.2, 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative
  State: Purified
  State: Lyophilized affinity purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.2 mg/ml (after reconstitution)
• Note: MHC class I antigens are heterodimers consisting of one α chain (44kDa) with β2-microglobulin (11.5 kDa). The epitope recognized by ER-MP42 is resistant to 0.05% glutaraldehyde, 1% paraformaldehyde and acetone.