MHC Class I H-2 (b,d,q,h4,m,w16) Rat Monoclonal Antibody [Clone ID: ER-HR52]

CAT#: BM4093

MHC Class I H-2 (b,d,q,h4,m,w16) rat monoclonal antibody, clone ER-HR52, Purified

Product Images

ER-MP42 and ER-HR52 anti H-2 monoclonal antibody reactivity

Specifications

Product Data

• Clone Name: ER-HR52
• Applications: Assay, FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200).
  Recommended Positive Control: Mouse Spleen.
  Does not react on routinely processed Paraffin Sections.
  Has been described to work in FACS.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse macrophage precursor cells
• Specificity: ER-HR52 is useful for detecting MHC class I antigens. It is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.
  The antigen is expressed by all somatic cells at varying levels. ER-HR52 detects MHC class I antigens of various haplotypes (see below).
  The antigen is found on all somatic cells in all organs sections though at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression.
• Formulation: PBS, pH 7.2 containing 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Concentration: 0.2 mg/ml
• Purification: Affinity Chromatography.
• Storage: Store the original vial at 2-8°C and the reconstituted stock solution (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Note: Protocol: Protocol with Frozen, ice-cold Acetone-Fixed Sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer’s hemalum.