MHC Class II (I-A k,b,d,q,r) Rat Monoclonal Antibody [Clone ID: ER-TR3]

CAT#: BM4094

MHC Class II (I-A k,b,d,q,r) rat monoclonal antibody, clone ER-TR3, Purified

Product Images

Immunohistochemistry: BM4094 MHC Antibody (Clone ER-TR3) staining of Mouse Spleen Frozen Section.

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  Distribution of ER-TR1, ER-TR2 and ER-TR3 among Mouse strains with independent and recombinant haplotypes: Percentage of labeled cells, determined by FACS analysis of spleen cell suspensions.

Specifications

Product Data

• Clone Name: ER-TR3
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1.5 µg/ml (1/200).
  Suggested Positive Control: Mouse spleen.
  Does not react on routinely processed paraffin sections.
  Has been reported to work in Flow Cytometry.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Murine thymic reticulum.
• Specificity: Monoclonal antibody ER-TR3 detects MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. it is a valuable tool for studying T-helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). This antibody also offers new possibilities for studying the development of T-helper cells since it also stains stromal cells in the thymus.
  Antigen Distribution
  Isolated Cells: The antigen is found on dendritic cells, B-cells and macrophages.
  Tissue sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.
• Formulation: Stock Solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as preservative
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.3 mg/ml
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C and reconstituted at -20°C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
• Note: Protocol: Protocol with Frozen, ice-cold Acetone-Fixed Sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum