MHC Class II (I-A k,b,d,q,r) Rat Monoclonal Antibody [Clone ID: ER-TR3]

CAT#: BM4094S

MHC Class II (I-A k,b,d,q,r) rat monoclonal antibody, clone ER-TR3, Purified

Product Images

Distribution of ER-TR3 among mouse strains with independent and recombinant haplotypes. * Percentage of labeled cells, determined by FACS analysis of spleen cell suspension

Specifications

Product Data

• Clone Name: ER-TR3
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Flow cytometry: The typical starting working dilution is 1:50.
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: The monoclonal antibody ER-TR3 reacts with mouse major histocompatibitity complex class II (MHC class II), also known as I-A or I-E.
  The monoclonal antibody ER-TR3 is a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells and macrophages) and for studying the development of T helper cells since they stain stromal cells in thymus. The level of antigen detected by ER-TR3 differs from strain to strain.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: 0.1 mg/ml
• Purification: Protein G
• Background: MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC Class II.