Endostatin (COL18A1) Mouse Monoclonal Antibody [Clone ID: 1837-46]

CAT#: BM4098

Endostatin (COL18A1) mouse monoclonal antibody, clone 1837-46, Purified


USD 655.00

2 Weeks*

Size
    • 200 ug

Product Images

Other products for "COL18A1"

Specifications

Product Data
Clone Name 1837-46
Applications IHC
Recommended Dilution Immunohistochemistry on Frozen Sections: 5-10 µg/ml (1/40-1/80).
Immunohistochemistry on Paraffin Sections: 5-10 µg/ml (1/40-1/80). Microwave pretreatment in citrate buffer is recommended for antigen retrieval in paraffin embedded tissues.
Recommended Positive Control: Human tonsil.
Reactivities Human
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Recombinant Human Endostatin (the carboxy-terminal domain)
Specificity This Monoclonal antibody Clone 1937-46 is directed against the Endostatin portion (the carboxy-terminal domain) of Collagen XVIII.
Formulation Stock solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as preservative
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 0.5 ml distilled water.
Concentration 0.4 mg/ml (after reconstitution)
Purification Affinity Chromatography
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens collagen type XVIII alpha 1 (COL18A1), transcript variant 1
Background Endostatin is a powerful inhibitor of endothelial cell proliferation and angiogenesis and shows widespread distribution, mainly in vessel walls and some other basement membrane zones.
Synonyms COL18A1, Endostatin, KNO1
Note Protocol: Protocol for Immunohistochemistry on paraffin sections:
1) Deparaffinize slides in chloroform for 30min and rehydrate to distilled water
2) Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0.01M citrate buffer pH 6.0.
3) Incubate slides for 15min in 200ml methanol/4.8ml 30%H2O2
4) Rinse slides 2x in distilled water and for 5min in TBS, pH 7.5
5) Block with normal swine serum (NSS, Jackson Immunoresearch #014-000-121) diluted 1:10 in Tris buffered saline pH 7.5, containing 0.1% bovine serum albumin (BSA, Calbiochem). Cover sections with NSS and incubate for 15min in a humid chamber at room temperature (RT).
6) Remove serum from sections (do not rinse)
7) Incubate with primary antibody diluted in TBS/0.1%BSA, pH 7.5 for 60min in a humid chamber at room temperature
8) Rinse sections in TBS, pH 7.5 for 5min, 2x
9) Incubate with secondary antibody (rabbit anti mouse IgG+IgM-biotin, Dako E 0464) 1:400 diluted in TBS/0.1%BSA, pH 7.5 for 30min in a moist chamber, room temperature. Preincubate now StreptABComplex /HRPO in TBS/0.1%BSA, pH 7.5!
10) Rinse sections in TBS, pH 7.5 for 5min, 2x
11) Incubate with StreptABComplex /HRPO (DAKO, K 0377) for 30min in a humid chamber at room temperature
12) Rinse sections in TBS, pH 7.5 for 5min, 2x
13) DAB-staining Prepare 3,3 - diaminobenzidintetrahydrochloride (DAB) according the manufacturers instructions (e.g. DAB tablets) and incubate slides for 1-15 min. Check color reaction to avoid overstaining.
14) Rinse sections in TBS, pH 7.5 for 5min, 2x
15) Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min
16) Mount slides with coverslips
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.