T Cells (Pan) Mouse Monoclonal Antibody [Clone ID: KI-T1R]
Specifications
Product Data | |
Clone Name | KI-T1R |
Applications | Assay, FC, IHC |
Recommended Dilution | Immunohistochemistry on Frozen Sections: 0.25 μg/ml (1/800). Ki-T1R shows very charateristic staining patterns in lymphoid organs according to the T-cell distribution; paracortical T-cell zone of lymphnodes, periarteriolar T-cell zones of the spleen, T-cell nodules of the gastrointestinal tract and bone marrow, thymocytes with prevalence from thymus medulla. This antibody has been described to work in FACS. |
Reactivities | Rat |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Rat lymphocytes. |
Specificity | This antibody reacts with Rat B-lymphocytes. This Monoclonal antibody Ki-T1R is a pan-T-cell marker useful for the characterisation of lymphoid organs and for the study of the interaction of T-cells with other cell types e.g. Macrophages. Ki-T1R detects an epitope of a 161kD glycoprotein (4 bands of 36kD, 38kD, 40kD and 47kD under reducing conditions) which is located on the outer surface of cytoplasmic membranes. Ki-T1R detects approximately 80% of peripheral blood T-cells. It is negative on all other cell types. |
Formulation | PBS, pH 7.2 with 0.01% Thimerosal as preservatives and 10 mg/ml BSA as stabilizer. Label: Biotin State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore with 0.5 ml distilled water. |
Concentration | 0.4 mg/ml |
Purification | Affinity chromatography. |
Conjugation | Biotin |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Background | Monoclonal antibody Ki-T1R is a pan-T-cell marker useful for the characterisation of lymphoid organs and for the study of the interaction of T-cells with other cell types e.g. macrophages. |
Synonyms | T-Cells, T Lymphocytes, T-lymphocytes |
Reference Data |
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