Cd4 Mouse Monoclonal Antibody [Clone ID: Rib 5/2]

CAT#: BM4101

Cd4 mouse monoclonal antibody, clone Rib 5/2, Purified

Product Images

Table 1. Comparison of CD4 and CD8 Monoclonal antibodies performed by Flow cytometry.

Specifications

Product Data

• Clone Name: Rib 5/2
• Applications: Assay, FC, FN, IHC
• Recommended Dilution: Suitable for Immunohistochemistry (IHC); has been described to work in FACS (see Table 1).
  Recommended Dilutions for IHC:
  Frozen sections: 1.5 μg/ml (1/200)
  Paraffin sections: does not react on routinely processed paraffin sections.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Lymphoblasts.
• Specificity: Recognizes a subpopulation of rat T-cells (T-lymphocytes and thymocytes, monocytes, macrophages).
  Rib 5/2 detects an epitope of the CD4 53kD glycoprotein on the cell surface. It is used for the identifcation of CD4 bearing cells, mainly T-helper / inducer lymphocytes.
  Rib 5/2 stains T-cell areas in the spleen and lymphnodes well. In the thymus Rib 5/2 detects cortical and medullary thymocytes. It recognises tissue macrophages and Th-cells according to the CD 4 distribution.
  It can also be applied for in vivo blocking of CD4 mediated immunological reactions.
• Formulation: PBS, pH 7.2 containing 0.01% Thiomerosal as preservative and 10 mg/ml BSA as stabilizer.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.3 mg/ml
• Purification: Affinity chromatography.
• Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Do not freeze working dilutions
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Cd4 molecule
• Database Link:
  Entrez Gene 24932 Rat
• Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
• Synonyms: T-cell surface antigen T4/Leu-3
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum