CD15 Mouse Monoclonal Antibody [Clone ID: VIM-C6]

CAT#: BM4104

CD15 mouse monoclonal antibody, clone VIM-C6, Purified

Specifications

Product Data

• Clone Name: VIM-C6
• Applications: CT, FC, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on frozen sections: 1 µg/ml (1:200) for tissue staining.
  Antigen Distribution:
  Isolated cells: VIM-C6 is heterogenously expressed on granulocytes and on monoblastoid precursor cells.
  Tissue sections: VIM-C6 is positive on myeloid precursor cells in the bone marrow. Cross reactions with glandular epithelial cells, astrocytes and Sternberg-Reed cells were reported for CD 15.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human granulocytes.
• Specificity: The antibody is directly cytotoxic with rabbit complement.
  VIM-C6 detects a carbohydrate sequence of the 3-fucosyl-N-acetyllactosamine.
• Formulation: PBS buffer pH 7.2 with 0.02% sodium azide as preservative and 10 mg/ml BSA as stabilizer.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.2 mg/ml
• Purification: Affinity chromatography.
• Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Do not freeze working dilutions
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: CD15 is a carbohydrate antigen expressed mainly on mature granulocytes and monocytes. CD15 is also called Lewis X, X-hapten, lacto-N-fucopentaose III or stage-specific embryonic antigen (SSEA). The reactivity is preferentially with granulocytes and to a lesser degree with monocytes. CD15 is expressed on immature bone marrow cells and leukemic cells of the myelo-monocytic lineage, and sometimes on lymphocytic leukemia cells. VIM-C6 facilitates the differentiation of granulocytes from other blood cells. It is also useful for phenotyping cell subpopulations in myeloic leukemias and for enrichment or elimination of myeloid cells. This antibody reacts with granulocytes, monoblastoid precursor cells.
• Synonyms: Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum