IL1RA (IL1RN) Mouse Monoclonal Antibody [Clone ID: 1384.92]

CAT#: BM4116B

IL1RA (IL1RN) mouse monoclonal antibody, clone 1384.92, Biotin

Specifications

Product Data

• Clone Name: 1384.92
• Applications: ELISA
• Recommended Dilution: ELISA: 2 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human IL-1Ra.
• Specificity: Monoclonal antibody 1384 is useful for studying biological effects of IL-1Ra in vitro.
  Reacts with IL-1 receptor antagonist.
  Negative with Human IL-1 beta, IL-1 alpha.
  Negative with Mouse IL-1Ra.
  Antigen Distribution: The antigen is produced and secreted by monocytes and macrophages after stimulation with pro-inflammatory cytokines, LPS, and in vitro by immunoglobulins coated to surfaces after an induction phase of 4h.
  In vitro the quantity of IL-1Ra secreted depends on the dose and type of stimulus.
• Formulation: Phosphate buffered saline, pH 7.2 containing 0.01% Thimerosal as a preservative and no stabilizer.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water (stock solution).
• Concentration: 0.4 mg/ml
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Do not freeze working dilutions
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: interleukin 1 receptor antagonist
• Database Link:
  Entrez Gene 3557 Human
• Background: The interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring molecule that shares homology with IL-1alpha and IL-1beta. It binds competitively to IL-1 receptors type I and type II with the same affinity as does IL-1, but does not induce any intracellular response. Two structural variants of IL-1Ra have been described: a 17kDa form that is secreted from monocytes, macrophages, neutrophils, and other cells (sIL-1Ra) and an 18-kDa form that remains in the cytoplasm of keratinocytes and other epithelial cells, monocytes, and fibroblasts (icIL-1Ra). An additional 16-kDa intracellular isoform of IL-1Ra has been described in neutrophils, monocytes, and hepatic cells. Both of the major isoforms of IL-1Ra are transcribed from the same gene through the use of alternative first exons. The production of IL-1Ra is stimulated by many substances including adherent IgG, other cytokines, and bacterial or viral components. The tissue distribution of IL-1Ra in mice indicates that sIL-1Ra is found predominantly in peripheral blood cells, lung, spleen, and liver, while icIL-1Ra is found in large amounts in skin. Studies in transgenic and knockout mice indicate that IL-1Ra is important in host defence against endotoxin-induced injury. IL-1Ra is produced by hepatic cells with the characteristics of an acute phase protein. Endogenous IL-1Ra is produced in numerous experimental animal models of disease as well as in human autoimmune and chronic inflammatory diseases. The use of neutralizing anti-IL-1Ra antibodies has demonstrated that endogenous IL-1Ra is an important natural antiinflammatory protein in arthritis, colitis, and granulomatous pulmonary disease.
• Synonyms: IL1F3, IL-1ra, IL-1RN, IRAP, IL1 inhibitor, ICIL-1RA