GFAP Mouse Monoclonal Antibody [Clone ID: GF-05]

CAT#: BM434

GFAP mouse monoclonal antibody, clone GF-05, Purified

Specifications

Product Data

• Clone Name: GF-05
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: reactive with antigen coated at 5 µg/ml (endpoint titer of a 1 mg/ml solution of this antibody is around 1/500,000).
  Western blot (recognizes 38-45 kDa neurofilaments).
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections and cell smears (0.1-10 µg per slide may be used as a guide).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Native GFAP purified from Human brain.
• Specificity: The antibody recognizes GFAP.
  It may react with GFAP from other species.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2670 Human
• Background: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
• Synonyms: Glial Fibrillary Acidic Protein