Histone H1 Mouse Monoclonal Antibody [Clone ID: AE-4]

CAT#: BM465S

Histone H1 mouse monoclonal antibody, clone AE-4, Purified

Specifications

Product Data

• Clone Name: AE-4
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western blotting.
  Immunoprecipitation.
  Immunofluorescence.
  Immunhistology on Frozen and Paraffin Sections: Acetone or PFA fixation is recommended for Frozen sections. Positive Control Tissue: Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A BALB/c mouse was immunized with Human leukemia biopsy cells.
  The isolated splenocytes were fused with mouse myeloma cells.
• Specificity: AE-4 recognizes Histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells. Tonsil is the recommended positive control tissue.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.05% Sodium Azide
• Concentration: 0.1 mg/ml
• Background: The histone proteins serve as a structural scaffold for the organisation of nuclear DNA into chromatin. In eucariotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that asseble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.
• Synonyms: H1 Histone Family, H1F4, H1F5, Histone H1a, Histone H1b