Adeno-Associated Virus 2 / AAV2 (intact particle) Mouse Monoclonal Antibody [Clone ID: A20]

CAT#: BM5010B

Adeno-Associated Virus 2 / AAV2 (intact particle) mouse monoclonal antibody, clone A20, Biotin

Specifications

Product Data

• Clone Name: A20
• Applications: ELISA, IF, IHC
• Recommended Dilution: ELISA: 1/20 for 1h at 37°C, use PBS with 0.05% Tween-20 as buffer.
  Neutralization Assay.
  Immunofluorescence Microscopy: Overnight at 2-8°C.
  Immunohistochemistry: Overnight at 2-8°C.
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Adeno-associated virus capsid proteins and virus particles
• Specificity: For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. Clone A20 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.
  The antibody cannot be used for Immunoblotting.
  Epitope mapping experiments (Wobus et al., see below) identified four immunoreactive (discontinous) regions.
  The major reaction was attributed to sequence aa 369 to 378 of AAV-2 capsids.
  The antibody is also useful for Neutralizing experiments (cf. Moskalenko et.al).
  In ELISA also Applicable to AAV-3.
• Formulation: Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.75 ml distilled water (Final solutin contains PBS with 0.09% Sodium Azide and 0.5% BSA)
• Concentration: 60 µg/ml (After reconstitution)
• Purification: Affinity Chromatography on Protein A
• Conjugation: Biotin
• Background: Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Serotype 2 (AAV2) has been the most extensively examined so far. AAV2 presents natural tropism towards skeletal muscles, neurons, vascular smooth muscle cells and hepatocytes.
• Synonyms: AAV-2