Adeno-Associated Virus 2 / AAV2 (VP1) Mouse Monoclonal Antibody [Clone ID: A1]

CAT#: BM5013

Adeno-Associated Virus 2 / AAV2 (VP1) mouse monoclonal antibody, clone A1, Purified

Specifications

Product Data

• Clone Name: A1
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunoblotting.
  Immunoprecipitation.
  Immunohistochemistry.
  Immunofluorescence Microscopy.
  Working Dilution: 1/5-1/10 for Immunohistochemistry.
  Dilution Buffer: PBS or Tris-buffered saline.
  Incubation Time. 3h at RT or overnight at 2-8°C for immunohistochemical application.
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Adeno-associated virus capsid proteins and virus Particles.
• Specificity: This Monoclonal Antibody A1 reacts with VP1 of AAV-2 found in Human and Monkey.
  Weak cross-reaction with serotypes 1, 3, 4, 5, 6.
  In Immunoprecipitation, an occasional reaction with a non AAV-derived protein is found.
  Epitope mapping experiments (See Ref.2) identified aa123 to aa131 of VP1 capsid protein as the specific binding region.
• Formulation: State: Purified
  State: Lyophilized purified IgG fraction with 0.09% Sodium Azide and 0.5% BSA
• Reconstitution Method: Restore with 1 ml dist. water
• Purification: Protein A Affinity Chromatography
• Background: Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. The capsid proteins: VP1, VP2 and VP3 interact together to form a capsid of an icosahedral symmetry.
• Synonyms: AAV-2