Adeno-associated Virus / AAV (VP1 + VP2 + VP3) Mouse Monoclonal Antibody [Clone ID: B1]

CAT#: BM5015

Adeno-associated Virus / AAV (VP1 + VP2 + VP3) mouse monoclonal antibody, clone B1, Purified

Specifications

Product Data

• Clone Name: B1
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1/10.
  Immunohistochemistry on Paraffin Sections: 1/10 (microwave treatment recommended).
  Western Blot: 1/250.
  (Dilute with PBS or TBS).
  Immunoprecipitation (mainly free VP proteins).
  Affinity Chromatography.
  Immunofluorescence Microscopy.
  
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Adeno-associated virus 2 capsid proteins and virus particles.
• Specificity: Useful for Immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. BM5010).
  B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm.
  Epitope mapping experiments (Wobus et al., see below) identified amino acids 726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region.
  The antibody is also useful for characterization of different stages of infection.
  Reacts with AAV-2, found in Human and Monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction, 11 &12 no cross-reaction).
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1 ml sterile PBS.
• Purification: Affinity Chromatography on Protein A (≥ 95% pure by SDS-PAGE)
• Synonyms: AAV1, AAV2, AAV3, AAV4, AAV5