Complement C3 (C3) Mouse Monoclonal Antibody [Clone ID: H206]

CAT#: BM5029

Complement C3 (C3) mouse monoclonal antibody, clone H206, Purified

Specifications

Product Data

• Clone Name: H206
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot.
  Immunohistochemistry on Frozen Sections: (also after Fixation with Paraformaldehyde).
  Working Dilution: 1/10 in PBS, pH 7.4.
  Incubation Time: 1h at RT.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human complement component C3
• Specificity: H206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kD fragment of C3c).
  Disorders Specifically Detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with Positive Control anti C3b-beta, (H11; Cat. No. BM2132) and Negative Control anti-C3a (H13; Cat. No. BM2131). Activated, deposited C3b bears C3b-alpha and C3b-beta, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H 13.
• Formulation: Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore in 1 ml distilled water
• Purification: Affinity Chromatography on Protein A
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement component 3 (C3)
• Database Link:
  Entrez Gene 718 Human
• Background: The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.
• Synonyms: CPAMD1, Complement component 3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus