Cytokeratin 5 (KRT5) (+ KRT8) Mouse Monoclonal Antibody [Clone ID: C22 (Ks 5+8.22)]

CAT#: BM5042

Cytokeratin 5 (KRT5) (+ KRT8) mouse monoclonal antibody, clone C22 (Ks 5+8.22), Purified

Specifications

Product Data

• Clone Name: C22 (Ks 5+8.22)
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytology.
  Immunohistochemistry on Frozen Tissues: 1/10.
  Incubation time: 1 h at RT.
  Immunohistochemistry on Paraffin-Embedded Tissues: 1/10.
  Protease pretreatment is required prior to antibody application.
  Incubation time: Overnight at 2-8°C.
  
  
  
• Reactivities: Amphibian, Bovine, Human, Hydra, Mouse, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human keratin K8, purified from SDS PAGE gel.
• Specificity: Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia.
  Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells.
  Epitope has been mapped to aa 353-367 on alpha helical rod domain (see Waseem et al., 2004).
  Reactivities on cultured cell lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.
  Epitope has been mapped to aa 353-367 on alpha helical rod domain (See Waseem et al. 2004).
• Formulation: Final solution contains 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer in PBS buffer, pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in 1 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens keratin 5 (KRT5)
• Database Link:
  Entrez Gene 110308 MouseEntrez Gene 3852 Human
• Background: Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
  
  Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
• Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5