Synaptopodin (SYNPO) Mouse Monoclonal Antibody [Clone ID: G1D4]

CAT#: BM5086

Synaptopodin (SYNPO) mouse monoclonal antibody, clone G1D4, Supernatant

Specifications

Product Data

• Clone Name: G1D4
• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections (after microwave treatment).
  Incubation Time: 1 h at RT for Immunohistochemistry .
  Working Dilution: Ready-to-use for Immunohistochemistry.
  Immunoblotting (Western Blot). The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
• Reactivities: Bovine, Guinea Pig, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Isolated Rat kidney glomeruli.
• Specificity: The antibody reacts specifically with Synaptopodin. In Human tissue Synaptopodin has a Molecular Weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in Mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
  1. The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells.
  2. Reacts with a subset of exclusively telencephalic synapses. Differentiationdependent expression during postnatal maturation of Rat brain. Differentiationdependent expression in cultured hippocampal neurons.
• Formulation: State: Supernatant
  State: Liquid Culture Supernatant
  Preservative: 0.01% Sodium Azide
• Database Link:
  Entrez Gene 11346 Human
• Background: Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities.
• Synonyms: KIAA1029