Collagen IV (COL4A1) Mouse Monoclonal Antibody [Clone ID: 1042]

CAT#: BM5124

Collagen IV (COL4A1) mouse monoclonal antibody, clone 1042, Ascites

Specifications

Product Data

• Clone Name: 1042
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunoblotting.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections (Proteolytic treatment required, Enzyme: Pepsin).
  Working Dilutions: 1/10-1/20
  Incubation Time: 1 h at RT
  Recommended Positive Control: Skin, kidney.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human placental Collagen type IV
• Specificity: Antibody 1042 is reactive with Human collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology.
  No crossreactivity with other Collagens.
• Formulation: PBS
  State: Ascites
  State: Liquid Ascites
  Preservative: 0.01% Sodium Azide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type IV alpha 1 (COL4A1), transcript variant 1
• Database Link:
  Entrez Gene 1282 Human
• Background: Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
• Synonyms: COL4A1

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer