DNA Mouse Monoclonal Antibody [Clone ID: AC-30-10]

CAT#: BM5520P

DNA mouse monoclonal antibody, clone AC-30-10, Purified

Specifications

Product Data

• Clone Name: AC-30-10
• Applications: IF, IHC
• Recommended Dilution: Cell Culture.
  Dot Blot on nitrocellulose membrane (after baking at 70°C).
  Immunohistochemistry on Frozen and Paraffin Embedded Tissue.
  Working Dilution: After reconstitution with 1 ml distilled water, dilute further 1/10 with PBS, pH 7.4 for Immunohistochemistry.
  Incubation Time: 1h at RT.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Double- and single-stranded DNA
• Specificity: Antibody clone AC-30-10 is a reliable Positive Control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases.
  It can also be used for sensitive detection of mycoplasma contamination in cell cultures.
  Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA.
  The antibody recognizes DNA in all DNA-containing species.
  Tested Reactivities on Cultured Cell Lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified IgM fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore in 1 ml distilled water
• Purification: Gel Filtration
• Background: dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
• Synonyms: Deoxyribonucleic Acid