OATP2 (SLCO1B1) (+ OATP8) Mouse Monoclonal Antibody [Clone ID: mMDQ]

CAT#: BM5541

OATP2 (SLCO1B1) (+ OATP8) mouse monoclonal antibody, clone mMDQ, Supernatant

Specifications

Product Data

• Clone Name: mMDQ
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Immunofluorescence Microscopy.
  Western blotting: For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37°C for 30 min (See also Ref.1).
  Immunohistochemistry on Paraffin Sections (Only after microwave treatment): Use undiluted.
  For details See Ref. 1.
  Incubation Time: 1 h at RT, for Paraffin Sections overnight at 2-8°C.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic N-terminus (24 aa) of Human organic anion transporter OATP2 coupled to KLH
• Specificity: In Western blot analyses of basolateral membrane fractions of human liver the monoclonal antibody mMDQ reacts with 90 kD and 120 kD proteins (after SDS-PAGE) representing OATP2 (also described as OATP-C) and OATP8, respectively. According to a recent new nomenclature these hepatocyte-specific proteins are identical to OATP1B1 and OATP1B3, respectively (See Ref.2).
  Using immunofluorescence microscopy mMDQ stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma (See Ref.1).
  Reacts with OATP2 and OATP8, human organic anion transporters (90 kD, 120 kD, after SDS-PAGE).
  Reacts with Transfected HEK293 cells; negative with HepG2 and PLC.
• Formulation: State: Supernatant
  State: Liquid Hybridoma Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Gene Name: Homo sapiens solute carrier organic anion transporter family member 1B1 (SLCO1B1)
• Database Link:
  Entrez Gene 10599 Human
• Synonyms: LST1, LST-1, OATP-2, OATPC, OATP-C, SLC21A6
• Note: Protocol: Western Blotting (Ref.1)
  1. For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended; alternatively, samples can be kept at 37°C for 30 min
  2.50 µg of protein lysate should be loaded on each lane.
  3. The usage of a PVDF-Membrane is recommended.
  4. The usage of 10% milk powder in TTBS (Tris-buffered saline, pH 7.6, with 0.05% Tween 20) as blocking reagent. The antibody can be diluted 3-5 x

Reference Data

• Protein Families: Druggable Genome, Transmembrane