Cytokeratin 5 (KRT5) (+ KRT8) Mouse Monoclonal Antibody [Clone ID: RCK102]

CAT#: BM6002P

Cytokeratin 5 (KRT5) (+ KRT8) mouse monoclonal antibody, clone RCK102, Purified

Product Images

RCK102 Cytokeratin staining of a human squamous cell carcinoma of the lung.

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  Immunohistochemistry on Frozen sections of human liver hepatocytes.

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  Immunohistochemistry on frozen sections of human liver hepatocytes and bile ducts

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  Immunohistochemistry on frozen sections of human urinary bladder epithelium

Specifications

Product Data

• Clone Name: RCK102
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/100-1/1000.
  Immunocytochemistry / Immunofluorescence: 1/10-1/500.
  Immunohistochemistry on Frozen Sections: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Tissues: 1/100-1/200.
  Flow Cytometry: 1/100-1/200.
  Recommended Dilutions: 1/100-1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 for Immunoblotting.
• Reactivities: Canine, Feline, Hamster, Human, Mouse, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Clone RCK102 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a Mouse immunized with Cytokeratins from a Human lung cancer cell line (MR21).
• Specificity: RCK102 is a Cytokeratin antibody reacting with Cytokeratin 5 and Cytokeratin 8.
  This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens keratin 5 (KRT5)
• Database Link:
  Entrez Gene 110308 MouseEntrez Gene 369017 RatEntrez Gene 3852 Human
• Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5