OB Cadherin (CDH11) Mouse Monoclonal Antibody [Clone ID: 16A]

CAT#: BM6020P

OB Cadherin (CDH11) mouse monoclonal antibody, clone 16A, Purified

Product Images

Western blot on a lysate of Cadherin 11 transfected PC3 cells

• 

  Immunocytochemistry on Cadherin 11 transfected PC3 cells

Specifications

Product Data

• Clone Name: 16A
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections: Use a PBS buffer containing 0.1 mM CaCl₂ and 0.1 mM MgCl₂.
  Recommended Dilutions: 1/25-1/50 for Immunohistochemistry with Avidin-biotinylated Horseradish Peroxidase Complex (ABC) as detection reagent and 1/25-1/250 for Immunoblotting applications.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Affinity purified extracellular domain of Human Cadherin-11-GST fusion protein.
• Specificity: This antibody recognizes the extracellular domain of Cadherin-11.
• Formulation: PBS with 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 1009 Human
• Background: Cadherins constitute a family of transmembrane glycoproteins involved in Ca²⁺-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.
• Synonyms: Osteoblast cadherin, OSF-4, CDH11, OB Cadherin