Integrin alpha 6 (ITGA6) (6B Isoform specific) Mouse Monoclonal Antibody [Clone ID: 6B4]

CAT#: BM6027P

Integrin alpha 6 (ITGA6) (6B Isoform specific) mouse monoclonal antibody, clone 6B4, Purified

Product Images

Immunostaining of Human ovarian carcinoma cells (cell line:OVCAR-3)

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  Frozen section of human kidney tissue immunostained with BM6027P.

Specifications

Product Data

• Clone Name: 6B4
• Applications: IF, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry on Frozen Sections.
  Recommended Dilutions: 1/50-1/100 for Flow Cytometry, and for Immunohistochemistry with Avidin-Biotinylated Horseradish Peroxidase Complex (ABC) as detection reagent, and 1/100-1/500 for Immunoblotting applications.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 6B4 is a mouse monoclonal IgG1, k antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a synthetic peptide corresponding to a 34 amino acid stretch in the cytoplasmic domain of integrin a6B including an appending N-terminal Cysteine coupled to keyhole limpet hemocyanin.
• Specificity: It recognizes specifically the cytoplasmic domain of integrin subunit alpha-6B.
  6B4 stains the basement membrane zone of ducts and acini of salivary gland, kidney tubules, epithelium of colonic crypts and weakly stains sweat glands and sebaceous glands.
  A broad species reactivity is expected because of the conserved nature of the epitope.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens integrin subunit alpha 6 (ITGA6), transcript variant 2
• Database Link:
  Entrez Gene 3655 Human
• Background: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated a and b subunits. More than 18 a and 8 b subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
  For integrin subunits a3 and a6, two cytoplasmic variants, A and B, have been identified.
• Synonyms: Integrin alpha-6, VLA-6, VLA6

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer