Sele (CR2 Region) Rat Monoclonal Antibody [Clone ID: UZ6]

CAT#: BM6034P

Sele (CR2 Region) rat monoclonal antibody, clone UZ6, Purified

Product Images

Immunostaining of E-Selectin transfected Chinese Hamster Ovary (CHO) cell.

Specifications

Product Data

• Clone Name: UZ6
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunoblotting.
  Immunoprecipitation.
  Flow Cytometry: 1/500.
  Immunofluorescence.
  Immunocytochemistry.
  Immunohistochemistry on Fozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
• Specificity: UZ6 recognizes a conformational epitope in the CR2 region in murine E-selectin.
  UZ6 partially inhibits leukocyte adhesion to murine activated endothelial cells.
• Formulation: PBS with 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Database Link:
  Entrez Gene 20339 Mouse
• Background: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
• Synonyms: SELE, ELAM1, LECAM2