Cardiotin Mouse Monoclonal Antibody [Clone ID: SR-2]

CAT#: BM6044P

Cardiotin mouse monoclonal antibody, clone SR-2, Purified

Specifications

Product Data

• Clone Name: SR-2
• Applications: IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin-Embedded Tissue.
  Recommended Dilutions: 1/25–1/100 for immunohistochemistry with ABC as detection reagent, and 1/50–1/500 for immunoblotting.
• Reactivities: Human, Porcine
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: 100 kDa Cardiotin subunit
• Specificity: This antibody SR-2 reacts with Cardiotin, a mitochondrionassociated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro.
  In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits.
  
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Background: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin.
  During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.