beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: B2M-01]

CAT#: BM604S

beta 2 Microglobulin (B2M) mouse monoclonal antibody, clone B2M-01, Purified

Product Images

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human bata2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line;Lane 2: EL4 mouse lymphoblastic lymphoma cell line;Lane 3: U937 human histiocytic lymphoma cell line

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  1. Murine 4T1 cells were mixed with human DU-145 cells and stained with 1 ug Beta-2-microglobulin antibody. Anti-mouse Cy3 was used as a secondary antibody. Red stained cells mark the Beta-2-microglobulin positive human DU-145 cells. 2. Murine 4T1 cells were stained with 1 ug Beta-2-microglobulin antibody. Anti-mouse Cy3 was used as a secondary antibody. Murine 4T1 cells are negative for Beta-2-microglobulin staining. Pictures by courtesy of Fraunhofer ITEM, Regensburg, Germany.

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  Confocal microscopy of Hman Beta-2-microglobulin-transfected canine cells labeled A) with antibody Alexa Fluor® 647 conjugate and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2 ug/ml.

Specifications

Product Data

• Clone Name: B2M-01
• Applications: ELISA, FC, IF, IHC, IP, R, WB
• Recommended Dilution: Flow Cytometry: 1 μg/ml.
  Positive Control: Peripheral blood lymphocytes.
  Negative Control: DAUDI Human Burkitt lymphoma cell line, erythrocytes.
  Immunoprecipitation: Clone B2M-01 coprecipitates beta-2-Microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of Human lymphoid cells.
  Western Blot: 2-4 μg/ml.
  Positive Control: U937 Human histiocytic lymphoma cell line.
  RAJI Human Burkitt lymphoma cell line.
  Negative Control: EL4 mouse lymphoblastic lymphoma cell line.
  Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
  Application Note: Both reducing and non-reducing conditions.
  Immunohistochemistry on Paraffin Sections: 10 μg/ml.
  Positive tissue: kidney glomeruli.
  ELISA: Working dilution should be determinated by investigator.
  RadioImmunoAssay: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10^-8 mol/l as determined by competitive RIA.
  Immunofluorescence: Customer feedback (see data below).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Human beta-2-Microglobulin
• Specificity: he antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.
  Negative Species: Mouse, Bovine, Canine (Dog), Rabbit and Chicken.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Sequential precipitation method with caprylic acid and ammonium sulphate
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 567 Human
• Background: Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
• Synonyms: B2M, Beta-2 microglobulin, Beta 2 microglobulin