Collagen III (COL3A1) Mouse Monoclonal Antibody [Clone ID: 8D1-8C7]

CAT#: BM8000

Collagen III (COL3A1) mouse monoclonal antibody, clone 8D1-8C7, Purified

Specifications

Product Data

• Clone Name: 8D1-8C7
• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA.
  RIA (> 1/1000).
  Immunflourescence assay: 1/80.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/1000
  Incubation Time: 60 min at RT or 2-8°C over night.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Positive Control: Human skin and placenta.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified collagen type III from human placenta
• Specificity: Human Collagen type III 100% (solid phase RIA at 1/500 dilution).
  Type III Collagen, MW 95 kDa, can be observed in skin, cartilage, human placenta and vitreous body.
  Cross Reactions:
  Human Collagen I, II, IV and V: < 0,1%
  Human Fibronectin and Laminin: < 0,1%.
• Formulation: PBS without preservatives or BSA.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore by adding 0.1 ml distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography.
• Storage: Store the antibody at 2-8°C for short term only.
  After reconstitution store and aliquot at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens collagen type III alpha 1 (COL3A1)
• Database Link:
  Entrez Gene 1281 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage, vitreous body, and human placenta.
• Synonyms: COL3A1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion