EPO Rabbit Polyclonal Antibody

CAT#: BP053

EPO rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: ELISA: 0.5-1 µg/ml.
  Western Blot: 1-2 µg/ml (Reducing and Non-Reducing Conditions).
  Functional Assays: The ND50 was determined to be approximately 1-3 µg/ml, in the presence of 0.3 units/ml of rhEpo, using a Human factor dependant cell line. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  Immunohistochemistry on Paraffin Sections: 15 µg/ml.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: CHO cell-derived, recombinant Human Erythropoietin.
• Specificity: This antibody reacts with Erythropoietin, the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 5% Trehalose
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2056 Human
• Background: Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
• Synonyms: Epoetin