Borrelia burgdorferi Rabbit Polyclonal Antibody

CAT#: BP1002F

Borrelia burgdorferi rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections. Acetone fixation of the antigen source is recommended prior to staining.
  Direct IFA staining of target antigens in a permissive tissue culture system.
  Western blot.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Whole cell preparation from B. burgdorferi.
• Specificity: Reacts with 83, 41, 34 and 31 kD proteins and their fragments in Western blots.
  Reacts also with T. pallidum, B. hermsii and B. parkerii.
• Formulation: 0.01M PBS, pH 7.2 with 10 mg/ml BSA as stabilizer and 0.09% sodium azide as preservative
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Highly purified Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: B. burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.
  B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.