Borrelia burgdorferi Rabbit Polyclonal Antibody

CAT#: BP1002HRP

Borrelia burgdorferi rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot.
  Immunocytochemistry.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Whole cell preparation from B. burgdorferi.
• Specificity: Reactive Western blot bands at 83 kD, 41 kD, 34 kD, 31 kD and additional low MW bands.
  Cross-reacts with Treponema pallidum, B. hermsii and B. parkerii.
• Formulation: PBS
  Label: HRP
  State: Liquid purified Ig fraction
  Stabilizer: 10 mg/ml BSA
  Preservative: 0.002% Thimerosal
  Label: Highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed
  Molar radio: Estimated molar HRP: IgG substitution is 2-3.
• Concentration: lot specific
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.
  B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.