VC1456 Rabbit Polyclonal Antibody

CAT#: BP1011HRP

VC1456 rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections (1:20 - 1:200).
  ELISA (1:200 - 1:1000).
  Western blot (1:20 - 1:200).
• Reactivities: Bacteria
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Choleragenoid.
• Specificity: This antibody detects the beta subunit of Cholera toxin.
• Formulation: Phosphate buffered saline, pH 7.2, containing 0.002 % Thimerosal and 1 % Bovine serum albumin
  Label: HRP
  State: Liquid purified Ig fraction
  Label: Horseradish Peroxidase
  Molar radio: 2-3
• Concentration: 1-2 mg/ml
• Conjugation: HRP
• Background: The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
• Synonyms: Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, ctxB, toxB, VC_1456