Collagen I (COL1A1) (+ type II, III, IV, and V) Rabbit Polyclonal Antibody

CAT#: BP1092P

Collagen I (COL1A1) (+ type II, III, IV, and V) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: ELISA (1/2000-1/8000).
  Immunoflourescence (1/10-1/40).
  Immunohistochemistry on Frozen Sections (1/10-1/40).
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A mixture of human collagen 1-5 from human placenta.
• Specificity: This antibody detects exhibits the following reactivities in ELISA both native and denaturated Collagen types:
  Collagen types 1 and 3: 100%.
  Collagen types 4 and 5: 90%.
  Collagen type 2: 80%.
  Human Fibronectin, Albumin and Immunoglobulins all < 0.5%.
• Formulation: PBS, pH 7.5 containing 0.1% Mannitol and 0.1% Dextran and 0.09% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on immobilized collagen types I-V
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type I alpha 1 chain (COL1A1)
• Database Link:
  Entrez Gene 1277 Human
• Background: Collagen I is a member of group I collagen (fibrillar forming collagen), it forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  Collagen II is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. High expression of isoform 2 in juvenile chondrocyte and low in fetal chondrocyte.
  Collagen III occurs in most soft connective tissues along with collagen I.
  Collagen IV is the major structural component of glomerular basement membranes, forming a Chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen.
  Collagen V is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Collagen V binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion