Apolipoprotein B (APOB) Goat Polyclonal Antibody

CAT#: BP2056HRP

Apolipoprotein B (APOB) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Can be used to detect the existence of Apo B-48/100 in plasma and lipoproteins for Immunoassay and Immunoblot.
  Dilution range for Immunoblot and ELISA: 1/2,500-1/10,000.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human LDL.
• Specificity: Binds to Human Apo B-48/100.
  No cross- reaction with human Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII and Apo E.
• Formulation: 50 mM PBS, 0.1M Sodium Chloride, pH 7.4 containing 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as preservative.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml distilled water.
  Centrifuge product if not completely clear after standing for 1-2 hours at room temperature.
• Concentration: lot specific
• Purification: Human Apolipoprotein B-100 Sepharose Affinity Chromatography.
• Conjugation: HRP
• Storage: Store the lyophilized antibody at 2-8°C.
  Store the antibody after reconstitution for several weeks at 2-8°C.
  Prepare working dilution only prior to immediate use.
  For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20°C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 338 Human
• Background: Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/l.
• Synonyms: ApoB, Apo-B, ApoB100, ApoB-100