Collagen I (COL1A1) Rabbit Polyclonal Antibody

CAT#: BP2156

Collagen I (COL1A1) rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: (SP)RIA.
  ELISA: 1/10-1/200.
  Immunofluorescence: In Indirect IFA with fluorescein anti-Rrabbit IgG conjugate, use diluted at 1/40 on Frozen salmon tissues (skin, liver).
  Immunostaining of extra or intracellular components in light microscopy and the quantitation of Salmon Type I collagen.
• Reactivities: Fish
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Collagen Type I extracted and purified from salmon fish skin.
• Specificity: Cross Reactivity: (OD at a 1/100 ELISA dilution).
  Salmon Fish Collagen Type I: ≥ 1.0
  Sole and Tuna fish Collagen Type I: ≤ 0.4
  Goldfish Collagen Type I: ≤ 0.2*
  Mammalian, avian Collagen Type I: ≤ 0.2*
  (*OD < 0.2 means negative reactivity).
• Formulation: State: Azide Free
  State: Lyophilized purified Ig fraction
  Preservative: None
• Reconstitution Method: Restore with 0.5 ml deionized water.
• Purification: Ion Exchange Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Should this product contain a precipitate we recommend microcentrifugation before use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type I alpha 1 chain (COL1A1)
• Database Link:
  Entrez Gene 1277 Human
• Background: Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion