Lipopolysaccharide (LPS gram negative bacteria) Goat Polyclonal Antibody

CAT#: BP2235

Lipopolysaccharide (LPS gram negative bacteria) goat polyclonal antibody, Purified

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Indirect Immunofluoresence: > 1/100 (against members of the Entero-bacteriaciae).
  May also be used in place of neat antiserum in almost any appropriate antibody-based technique.
  Also suitable for conjugation purposes.
• Reactivities: Enterobacter
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Whole cells prep of Lipid A from E. coli O157
• Specificity: The antibody recognizes Lipid A / Lipopolysaccharide (LPS Gram Negative Bacteria).
  Cross-reactive with numerous members of the Enterobacteriaciae: Pseudomonas aeruginosa, Klebsiella pneumoniae, E. coli O157, Salmonella enteriditis, Enterobacter aerogenes, E. hermanii, Yersinia enterocolitica and Shigella sonnei.
• Formulation: 0.01M PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure)
  Stabilizer: None
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Sodium Sulphate Precipitation and Ion Exchange Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
• Synonyms: Lipid A, LPS