MRP8/14 (S100A8/A9) Chicken Polyclonal Antibody

CAT#: BP4011

MRP8/14 (S100A8/A9) chicken polyclonal antibody, Aff - Purified

Product Images

BP4011 S100A8/A9 antibody staining of Human Skin Frozen Section.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on Frozen Sections: 0.5 µg/ml (1/2000).
  Immunohistochemistry on Paraffin Sections: 4 µg/ml (1/250). Proteinase K pretreatment for antigen retrieval is recommended.
  Recommended Positive Control: Human tonsil.
• Reactivities: Bovine, Human, Porcine
• Host: Chicken
• Clonality: Polyclonal
• Immunogen: Recombinant MRP8 and MRP14
• Specificity: This polyclonal antibody recognizes different epitopes on the MRP8/14 heterocomplex (S100A8/S100A9). It is particularly suited for use in ELISA or similar assays. It was generated by immunising chickens with recombinant MRP8/14. Thus, the antibody does not discriminate between the individual subunits MRP8 or MRP14.
  In Immunohistochemistry, it detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears. The MRP8/14 specific ELISA has shown significant advantages in the early assessment of certain acute inflammatory
  conditions.
  Antigen Distribution on Tissue Sections: Macrophages in the red pulp of the spleen and in the liver; strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen; absent on normal resident mononuclear phagocytes in healthy tissues (skin, gut, thymus).
• Formulation: PBS with 0.09% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified IgY fraction from Egg Yolk
• Concentration: 1.0 mg/ml
• Purification: Affinitty Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Do not freeze working dilutions
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Synonyms: Calgranulin A/B, Calprotectin, L1 Protein, CFAG, P8, P14, CAGA, CAGB, CFAG, MRP-8, MRP-14