Cytokeratin 8 (KRT8) (+ KRT18) Guinea Pig Polyclonal Antibody

CAT#: BP5007

Cytokeratin 8 (KRT8) (+ KRT18) guinea pig polyclonal antibody, Serum

Product Images

IHC staining of rat liver (courtesy of J.Heß, University Hospital Heidelberg).

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  IHC staining of rat colon (courtesy of J.Heß, University Hospital Heidelberg).

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  IHC staining of rat kidney (courtesy of J.Heß, University Hospital Heidelberg).

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  IHC staining of mouse kidney (courtesy of J.Heß, University Hospital Heidelberg).

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  IHC staining of mouse colon (courtesy of J.Heß, University Hospital Heidelberg).

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  Western blot with human cell lines using anti Cytokeratin 8/18 antibody BP5007.

• Western Blot using anti Cytokeratin 8/18 antibody BP5007 at 1/2000. MCF7 whole cell lysate (1.3 - 10 µg).

• Immunofluorescence staining of mouse CDβGeo cells with anti-Keratin K8/18 (green), Keratin K5 (Covance, red) and Dapi (blue) (Courtesy of Karen A Dunphy, Veterinary and Animal Sciences, University of Massachusetts).

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Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunoblotting: 1/2000 for Western blotting.
  Cytological material.
  Immunohistochemistry on Frozen Tissue: 1/100.
  Immunohistochemistry on Paraffin-Embedded Tissue: 1/50 (microwave treatment recommended).
  Incubation Time: 1 h at RT, extended with Paraffin.
• Reactivities: Bovine, Chicken, Hamster, Human, Mouse, Rat, Xenopus
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Keratin K8/K18 filaments, reconstituted from purified Bovine keratins K8 and K18
• Specificity: Reactive Polypeptide: Mr 52 500 and 45 500 Keratins K8 and K18 of simple type epithelium.
  Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma.
• Formulation: State: Serum
  State: Liquid stabilized antiserum
  Preservative: 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens keratin 8 (KRT8), transcript variant 2
• Database Link:
  Entrez Gene 16691 MouseEntrez Gene 25626 RatEntrez Gene 3856 Human
• Background: Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
• Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A

Reference Data

• Protein Families: Druggable Genome