Cytokeratin 9 (KRT9) Guinea Pig Polyclonal Antibody

CAT#: BP5008

Cytokeratin 9 (KRT9) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunoblotting: 1/5000-1/10,000 for Western Blot (ECL).
  Immunohistochemistry on Frozen Sections: 1/100-1/200.
  Immunohistochemistry on Paraffin Embedded Sections: 1/50 (Microwave retrieval method is recommended).
  Incubation Time: 1 h at RT, extended with Paraffin.
• Reactivities: Bovine, Hamster, Human, Mouse, Rat, Xenopus
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: KLH conjugated Synthetic peptide corresponding to amino acids 450-477 of Human keratin K9
• Specificity: The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia.
  Negative tissues include: muscle, liver and duodenum (See references 1 and 2).
• Formulation: State: Serum
  State: Liquid Serum
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3857 Human
• Background: Acidic keratin K9 (MW 62,129; formerly also designated cytokeratin 9), expressed in the palmoplantar epidermis.
• Synonyms: Cytokeratin 9, KRT9, CK9, Keratin-9, K9