ADFP (PLIN2) (N-term) Guinea Pig Polyclonal Antibody

CAT#: BP5012

ADFP (PLIN2) (N-term) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunoblotting (Western blot): 1/2000.
  Immunohistochemistry on Frozen Sections: 1/100 (See Ref.11 for staining protocols).
  Immunohistochemistry on Paraffin Sections: 1/100 using microwave treatment (See Ref.13 for staining protocols).
  Cytological Material.
  Incubation Time: 1 h at RT or overnight at 2-8°C.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide (N-terminal aa 1-29 of Human and Murine Adipophilin).
• Specificity: Specific for Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDSPAGE); pI 6.72.
  Tissue Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages.
  Tested Reactivities on Cultured Cell Lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (Human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK.
  Negative with glioma.
• Formulation: State: Serum
  State: Whole Antiserum
  Preservative: 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 123 Human
• Background: Adipophilin / ADRP (a member of the PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.; for review see e.g. Targett-Adams et al.); inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (see e.g. Hope et al.).
• Synonyms: Adipose differentiation-related protein, ADRP