Plectin (PLEC) (C-term) Guinea Pig Polyclonal Antibody

CAT#: BP5019

Plectin (PLEC) (C-term) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot (1/16,000 using ECL).
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections (1/500).
  Incubation Time: 1 h at RT, extended with paraffin sections; Microwave treatment enhances staining on paraffin sections.
• Reactivities: Fish, Human, Xenopus
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: C-terminal domain of Human recombinant Plectin (aa. 4367-4684).
• Specificity: Calculated MW 532,000 protein plectin, localizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmo- somes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle.
  Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes).
• Formulation: State: Serum
  State: Liquid whole antiserum
  Preservative: 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5339 Human
• Background: Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking. Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes. Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions.
• Synonyms: PLEC, Plectin-1, PLEC1, PCN, PLTN