Gastrin (GAST) Guinea Pig Polyclonal Antibody

CAT#: BP5046

Gastrin (GAST) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Tissues (No proteolytic treatment required).
  Immunofluorescence Microscopy.
  Recommended Positive Control: Formalin-fixed paraffin sections of Rat antrum.
  Working Dilutions: 1/800-1/1500 using FITC, with overnight incubation at 2-8°C.
• Reactivities: Human, Rat
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic Human Gastrin I conjugated to BSA
• Specificity: Gastrin-secreting cells are numerous in the antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of Gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum.
  Absorption with 10-100 µg Gastrin 1-34 and CCK 8 per ml antiserum abolishes the staining.
• Formulation: State: Serum
  State: Lyophilized Serum
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Dissolve the antiserum in 50-100 µl distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.09% Sodium Azide.
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 2520 Human
• Background: Gastrin is mainly known by its first described role as a gastric acid-hormone but the protein also has the ability to promote cellular growth. More recently, gastrin has been suggested to induce leukocyte-endothelial cell interactions and to have a pro-inflammatory effect. Gastrin has two biologically active peptide forms, G34 and G17 and they activate two different receptors: the CCK-1 receptor, which has low affinity for gastrin but high affinity for the related hormone cholecystokinin (CCK), and the CCK-2 receptor, which has high affinity for both gastrin and CCK and mediates the acid-secretory as well as the proliferative effects of gastrin.
• Synonyms: GAST, GAS