Gastrin (GAST) Guinea Pig Polyclonal Antibody

CAT#: BP5046

Gastrin (GAST) guinea pig polyclonal antibody, Serum


USD 395.00

2 Weeks*

Size
    • 50 ul

Product Images

Specifications

Product Data
Applications IF, IHC
Recommended Dilution Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Embedded Tissues (No proteolytic treatment required).
Immunofluorescence Microscopy.
Recommended Positive Control: Formalin-fixed paraffin sections of Rat antrum.
Working Dilutions: 1/800-1/1500 using FITC, with overnight incubation at 2-8°C.
Reactivities Human, Rat
Host Guinea Pig
Clonality Polyclonal
Immunogen Synthetic Human Gastrin I conjugated to BSA
Specificity Gastrin-secreting cells are numerous in the antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of Gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum.
Absorption with 10-100 µg Gastrin 1-34 and CCK 8 per ml antiserum abolishes the staining.
Formulation State: Serum
State: Lyophilized Serum
Preservative: 0.09% Sodium Azide
Reconstitution Method Dissolve the antiserum in 50-100 µl distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.09% Sodium Azide.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Background Gastrin is mainly known by its first described role as a gastric acid-hormone but the protein also has the ability to promote cellular growth. More recently, gastrin has been suggested to induce leukocyte-endothelial cell interactions and to have a pro-inflammatory effect. Gastrin has two biologically active peptide forms, G34 and G17 and they activate two different receptors: the CCK-1 receptor, which has low affinity for gastrin but high affinity for the related hormone cholecystokinin (CCK), and the CCK-2 receptor, which has high affinity for both gastrin and CCK and mediates the acid-secretory as well as the proliferative effects of gastrin.
Synonyms GAST, GAS
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.