KRT13 Guinea Pig Polyclonal Antibody

CAT#: BP5076

KRT13 guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/3000 (ECL method).
  Immunohistochemistry on Frozen Sections: 1/200.
  Immunohistochemistry on Paraffin Sections: 1/100 (after microwave treatment).
  Immunohistochemistry on Cytological Material.
  Positive Control: Squamous epithelium of esophagus, gingival, palate, vagina (staining all of the suprabasal layers).
  Incubation Time: 1h at RT, extended with Paraffin Sections.
• Reactivities: Human, Mouse
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide (TTTSSASVTTTSNA-C) of Human keratin K13 (formerly also designated cytokeratin 13), coupled to KLH.
• Specificity: This antibody recognizes Acidic Human keratin K13 (Mr 55 000).
  Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin. Negative on foot sole, scalp.
  Tumors Specifically Detected: Several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma.
• Formulation: State: Serum
  State: Stabilized Liquid antiserum
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 13
• Database Link:
  Entrez Gene 3860 Human
• Background: Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.
• Synonyms: CK-13, CK13, Keratin-13, KRT13, K13, KRT-13