Cytokeratin 15 (KRT15) Guinea Pig Polyclonal Antibody

CAT#: BP5077

Cytokeratin 15 (KRT15) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence Microscopy on Frozen Sections.
  Immunohistochemistry on Paraffin Sections (after microwave treatment or Trypsin).
  Working Dilution: 1/100-1/200 for Immunohistochemistry.
  Incubation Time: 1h at RT.
  Positive Control: Sweat glands of human skin (e.g. foot sole).
• Reactivities: Human, Mouse
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human Keratin K15 (formerly also designated cytokeratin 15), coupled to KLH.
• Specificity: MW 50,000 (pI 4.9) intermediate filament polypeptide, keratin K15, detected by Immunohistochemistry in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. bronchial epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the basal cell layer of the outer root sheath.
  Completely non-reactive were cells of liver, colon.
• Formulation: State: Serum
  State: Liquid Serum
  Preservative: 0.09% Sodium Azide
• Storage: Store the antibody undiluted at 2-8°C.
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3866 Human
• Background: Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.
• Synonyms: Cytokeratin-15, CK-15, K15, Keratin-15, Keratin 15, KRTB, KRT15