Drebrin (DBN1) (324-343) Guinea Pig Polyclonal Antibody

CAT#: BP5113

Drebrin (DBN1) (324-343) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunofluorescence / Immunocytochemistry (assay dependent).
  Immunoblotting (assay dependent).
  Immunoprecipitation (assay dependent).
  Immunohistochemistry on Frozen Paraformaldehyde-Fixed Sections: 1/100.
  Immunohistochemistry on Paraffin Sections: 1/100 (Microwave treatment is recommended)
  Incubation Time: 1h at RT, extended with paraffin.
  Immunohistochemistry on Cytological Material.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic Human peptide (aa 324-343) coupled to KLH.
• Specificity: The antiserum specifically reacts with Drebrin isoforms E2 and A, widespread actin-associating proteins of 70 kD (SDS-PAGE mobility shows Mr 120,000).
  Reacts with PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80; B1; B16 (Mouse melanoma cells).
• Formulation: State: Serum
  State: Liquid Serum
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1627 Human
• Background: Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.
• Synonyms: DBN1, DBN-1, D0S117E, DKFZp434D064,drebrin 1