COL1A1 Rabbit Polyclonal Antibody

CAT#: BP8000S

COL1A1 rabbit polyclonal antibody, Purified


USD 490.00

2 Weeks*

Size
    • 100 ul

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Specifications

Product Data
Applications ELISA, IF, IHC, R
Recommended Dilution RIA.
ELISA: 1/400 (OD ≥ 500).
Immunoblotting (Western blot).
Immunofluorescence (1/80).
Immunohistochemistry on Paraffin embedded tissues: 1/100-1/500. 
Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Incubation Time: 60 min at RT or 2-8°C overnight.
Positive Control: Bovine skin.
Reactivities Bovine
Host Rabbit
Clonality Polyclonal
Immunogen Purified Collagen type I from Bovine skin.
Specificity Bovine Collagen type I: 100%.
Bovine Collagen II, IX, XI: < 0.1%
Sheep Collagen type I: 50% (IHC estimated)
Bovine Fibronectin: < 0.1%
Human Collagen type I: < 2%
Chicken Collagen type I: < 0.1%
(Estimates except Sheep Collagen I from solid phase RIA at dilution 1/500).

Even though cross-reactivity with Human is low in RIA, the antibody has to be expected to be cross-reactivive to Human Collagen type I as well as to Pig Collagen type I in IHC.

Formulation PBS without BSA and preservatives
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: None
Preservative: None
Reconstitution Method Restore with 0.1 ml (BP8000S) or 0.5 ml (BP8000) distilled water.
For further dilution use appropriate antibody diluent.
Concentration ~1.0 mg/ml (after reconstitution)
Purification Affinity Chromatography
Background Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type I Collagen (95 kDa) usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues.
Synonyms COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.