Collagen I (COL1A1) Rabbit Polyclonal Antibody

CAT#: BP8004

Collagen I (COL1A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: IF: 1:40
  IHC(P): 1:200 - 1:600; 60 min at RT or 2-8°C over night.
  ELISA.
  RIA.
  Positive control: goldfish skin.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Fish, Goldfish
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type I from goldfish skin
• Specificity: This antibody is specific for collagen type I.
• Formulation: Phosphate buffered solution; no BSA and preservative added
  State: Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water, for further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type I alpha 1 chain (COL1A1)
• Database Link:
  Entrez Gene 1277 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion