Collagen I (COL1A1) Rabbit Polyclonal Antibody

CAT#: BP8007

Collagen I (COL1A1) rabbit polyclonal antibody, Purified

Product Images

Collagen Type I on porcine skin paraffin section. [CO20191], DAB, hematoxylin

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA.
  ELISA: ≥ 1/200 shows OD ≥ 500.
  Immunofluorescence: 1/80 (1/500 immunoperoxidase ABC or labelled polymer-technic).
  Immunohistochemistry on Frozen and Paraffin Embedded Sections: ≥ 1/500.
  Incubation Time: 60 min at RT or 2-8°C over night.
  Positive Control: Porcine skin and tendon.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type I from Porcine tendon.
• Specificity: This antibody is specific to Porcine Collagen type I.
  Cross reactivity:
  Collagen type III: < 1%
  Collagen type II, IV, V: < 0.1%
  Elastin: < 0.1%
  Fibronectin < 0.1%
  Cross reactivity with Rat and Bovine < 1%, Human and Mouse < 0.1%.
• Formulation: PBS without BSA or preservatives
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.1 ml distilled water, for further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type I alpha 1 chain (COL1A1)
• Database Link:
  Entrez Gene 1277 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for crosslinkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion